The application of thermophilic DNA primase TtDnaG2 to DNA amplification
نویسندگان
چکیده
منابع مشابه
Evolution of Thermophilic DNA Polymerases for the Recognition and Amplification of C2’-Modified DNA
The PCR amplification of oligonucleotides enables the evolution of sequences called aptamers that bind specific targets with antibody-like affinity. However, in many applications the use of these aptamers is limited by nuclease-mediated degradation. In contrast, oligonucleotides that are modified at their sugar C2' positions with methoxy or fluorine substituents are stable to nucleases, but the...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-12241-6